Volume 42 - Issue 3 - 323 - 330

Use of poly(HEMA-MAH)-Cu2+ Microbeads for α-Amylase Immobilization

Poli(HEMA-MAH)-Cu2+ Mikrokürelerin α-Amilaz İmmobilizasyonu için Kullanılması

In this article poly(2-hydroxyethyl methacrylate-N-methacryloyl-(l)-histidin methylester) [P(HEMA-MAH)] microbeads were prepared by suspension polymerization of 2-hydroxyethyl methacrylate (HEMA) and N-methacryloyl-(l)-histidine methyl ester (MAH). Some properties of the p(HEMA-MAH) microbeads were determined by using scanning electron microscopy (SEM) and swelling tests. Cu2+ ions were chelated on the p(HEMA-MAH) microbeads, then these microbeads were used in the immobilization of α-amylase in batch system. The maximum α-amylase adsorption capacity of the p(HEMA-MAH)-Cu2+ beads was found to be 14.88 mg/g at pH 6.0. The optimum temperatures for the immobilized and free enzyme were determined to be 30oC and 25 oC, respectively. Kinetic parameters (Km, Vmax) of the immobilized enzyme were also determined and compared with those of free enzyme. Reuse studies showed that the immobilized enzyme could reuse six times while retaining 76% of its activity. 

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