Helichrysum species (Asteracea) are widely found in Anatolia. Helichrysum species has used ethnomedicine for centuries. We report here the total phenolic contents and antioxidant activities of the methanolic extracts of Helichrysum plicatum DC. subsp. plicatum species, together with their HPLC analysis results of individual some phenolic acids and flavonoids. 17 different phenolic constituents were measured by reverse phase- high performance liquid chromatograpy (RP-HPLC) in the three parts of the plant. Total phenolic compound and ferric reducing antioxidant power (FRAP) were used as antioxidant capacity determinants. All parts of the plants showed high antioxidant activity containing large amounts of antioxidant compounds. Chlorogenic acid, quercetin and rutin found in the three parts of the samples as main phenolic components and absisic, ferulic, epicathecin and cinnamic acids have minor concentration or haven’t been detected at all. The methanolic extracts of the plants proved to be a good source of phenolic compounds and antioxidants agents that might serve to protect health and fight against several diseases.