Volume 42 - Issue 4 - 563 - 568

Purification of Lysozyme with Congo Red Immobilized Cryogel Discs

Kongo Kırmızısı İmmobilize Kriyojel Disklerle Lizozim Saflaştırılması

In this study, congo red immobilized poly(hydroxyethyl methacrylate) [CR-PHEMA] cryogel discs were pre- pared for purification of lysozyme. CR-PHEMA cryogel discs were characterized by swelling test, elemental analysis and scanning electron microscopy. Lysozyme adsorption was performed from aqueous solutions and maximum lysozyme adsorption capacity of CR-PHEMA cryogel discs was found to be 136.6 mg/g. Effects of initial lysozyme concentration, pH and ionic strength on lysozyme adsorption capacity of CR-PHEMA cryogel discs were investigated. Maximum adsorption was obtained at pH 8.0. In the last step of this study, lysozyme was purified from egg white using CR-PHEMA cryogel discs. Egg white fractions were observed on sodium do- decyl sulfate polyacrylamide gel electrophoresis. 

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