Plasma fractionation is the first process for large scale protein purification developed about 60 years ago by Cohn and co-workers. Currently therapeutically important proteins are human serum albumin (HSA), immunoglobulin G (IgG), coagulation factor VIII and protease inhibitors. HSA is one of the most abundant proteins in plasma and, together with IgGs, constitutes 80% of all plasma proteins. According to the data, HSA represented approximately 50% of all sales of therapeutic plasma protein products. The purification of HSA is generally required for the treatment of hypoproteinemia and heavy loss of blood, requires relatively high purity for medical use. HSA also used for therapeutic purposes such as shock, burns, hypoalbuminemia, surgery, trauma, cardiopulmonary bypass, the acute respiratory distress syndrome, hemodialysis, acute nephrosis, hyperbilirubinemia, acute liver failure, acites, and sequestration of protein-rich fluids in acute peritonitis, pancreatitis, mediastinitis and extensive cellulites.