This paper deals with the floristic study of Truva National Park (Çanakkale/Turkey). Thus floristic specializa- tion of this area described, including a number of endemic and threatened species. After identification of the specimens, total flora has been determined as 361 taxa belonging to 354 species, 245 genera and 70 families. The endemism rate of the area is 3.32% (12 taxa). In a study of the endangered flora and its protection, 15 taxa are listed as having the most restricted distribution and as being under threat of extinction. Field work for these taxa was undertaken in order to improve knowledge of their habitats, populations, chorology and main threats. Using the new IUCN categories of threat, an analysis was made for each of the taxa, especially those that are critical endangered with very low number of individuals or very narrow areas of occupancy. In order to conserve the biological diversity of Truva, the need for more nature reserves, especially in the Yenikumkale, Orhaniye and Truva region is highlighted. A number of key strategies for protection are proposed.