Volume 37 Issue 2


 Dear readers of the Hacettepe Journal of Biology and Chemistry,

Progress of science demands communication. Once, oral conservations and letters were the only tools for transmitting knowledge between scientists, modern technological developments and the explosive growth of the number of scientists have made possible and necessitated global communication by means of papers in hard copy and electronic journals.

From Capillary Electrophoresis to Capillary Electrochromatography: An Overview
77 - 82
Identification of Genotypes of Cryptosporidium parvum in Children with Diarrhea by PCR-RFLP

Various Cryptosporidium species have been associated with human and animal infections, especially C. parvum infects primarily immunocompromized individuals and causes chronic or acute diarrhea. Outbreaks of human cryptosporidiosis are often cause by the contamination of water supplies with this parasite throughout the world. Because the epidemiological importance it is desirable to develop a sensitive detection technique for this parasite and accurate genetic classification. In this study, we collected samples of whole feces from 256 children with diarrhea at Social Security Children’s Hospital, Ankara, Turkiye. For detecting the oocysts in feces, we used modified acid-fast staining method and direct immunofluorescent assay. For molecular studies, a fragment of the Cryptosporidium COWP and SSU rRNA genes encompassing the hypervariable region was amplified by PCR and then RFLP analysis was performed for distinguishing the Cryptosporidium species in the samples. In RFLP analysis Rsa I, Ssp I and Vsp I enzymes were used to identify the species/genotypes of Cryptosporidium. In the samples studied, we have determined only Cryptosporidium parvum and its two genotypes. The prevalance of Cryptosporidium positivity was about 4.7%.

83 - 91
Synthesis and Characterization of Poly(3,4-Diacetamidostyrene)
93 - 101
Optimization of Separation of Several Sulfonamides in Acetonitrile- Water Binary Mixtures by Using Symmetry Shield RP-8 Column
103 - 110
Effects of Some Antibiotics on Enzyme Activities of Carbonic Anhydrase from Erythrocytes in Vivo and in Vitro
111 - 122
The Bio-Stimulating Effects of Invasive Caulerpa Var. cylindracea Extract on Vignia sinensis and Phaseolus vulgaris (Fabaceae)
123 - 138
The Concentration and Separation of Cu2+, Ni2+, Fe3+ Ions by Complexation with Poly(ethyleneimine) and Using A Suitable Membran
139 - 144
Synthesis and Characterization of Hydrogels Formed from Glycidyl Methacrylate (GMA) with Anhydride Derivatives
145 - 155
Poly(hydroxyethyI methacrylate) Nanoparticles for Environmental Applications
157 - 168
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