Volume 42 - Issue 4 - 509 - 515

Human IgG Adsorption by Spherical Albumin Biosorbents in SBF Media

Küresel Albumin Biyosorbentlerle SBF ortamlarında İnsan IgG Adsorpsiyonu

The objective of this study was to investigate the removal efficiency of immunoglobulin G (IgG) by albumin bi- osorbents in batch wise in four different simulated body fluid (SBF) solutions at room temperature. Albumin biosorbents were prepared as microsphere form by emulsion polymerization method. 0.1 g/ml albumin concentration, 1000 rpm stirring rate, 1% glutaraldehyde (GA) concentration 30 minutes crosslinking time were determined as optimal conditions in our previous study. The prepared biosorbents were then studied for IgG removal efficiency in four simulated body fluid (SBF) solutions [N(Normal)-SBF, G(glucose)-SBF, H(hepes)-SBF, T(tris)-SBF] at room temperature. IgG capturing capacity was obtained as 320 mg IgG/g with albümin biosor- bent (94,6%) in N-SBF. It was observed that there was no leakage at washing with water. It may be concluded that prepared albumin biosorbents are sufficient in terms of efficiency of IgG removal. It was seem that this method is easy to perform and is useful as a first step in the detection of diagnostic markers in body fluids by applying proteomics technologies. We believe that albumin biosorbents offered the promising approach with good removal specificity and efficiency of IgG. 

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