Proteins are essential parts of organisms and participate in every process within cells. Protein biosensors are becoming more essential for the through and systematic investigation of complex biological processes. Several methods have been used for development of protein biosensors e.g., surface plasmon resonance, quartz crystal microbalance, chemiluminescence, electrophoresis, fluorescence techniques and electrochemical methods. There has been growing interest for electrochemical metods in protein research (like protein-protein interactions, or some diseases related to proteins investigations). Herein, this paper will specifically focus on new trends in electrochemical protein sensors.