A series of poly(vinyl alcohol) (PVA)/poly(vinyl pyrrolidone) (PVP)/poly(ethylene glycol) (PEG) hybrid nanofibers were fabricated by an electrospinning method, in order to be used as a potential wound dressing material. The effect of Aloe vera was investigated in terms of solution properties such as viscosity, conductivity, pH and surface tension. The nanofibrous wound dressing materials were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) and differential scanning calorimetry (DSC). SEM results revealed that the fiber diameters ranged between 200-500 nm and they were linear, homogenous and free of beads. DSC thermograms indicated that the addition of Aloe vera affects the crystal structure and efficiently cross-links the hybrid polymer, forming an amorphous structure with a melting temperature (Tm) of 219oC at an Aloe vera concentration of 3%. FTIR results clearly resolved the hybrid polymer structure of nanofibers obtained.