The Cytotoxicity Evaluation of Melia azedarach L. Extracts on Human Adipose-derived Mesenchymal Stem Cells

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Burcu Efe Yusuf Furkan Galata Yavuz Emre Arslan http://orcid.org/0000-0003-3445-1814

Abstract

Medicinal plants are an important source and used for several crucial medicinal treatments. Due to their potential biological properties, their extracts have been studied intensively. In this study, aqueous extracts of Melia azedarach L. green fruit and leaves were obtained using two different extraction methods. The extraction yield of the green fruit and leaves was found as 24.11% and 37.98% for the infusion method; 17.76% and 27.00% for the rotating method, respectively. Total phenolic content and antioxidant activities were also measured via spectrophotometric techniques. The total phenolic content, related to the infusion method, was ascertained for green fruit extract 173.67 ± 10.84 mg Gallic Acid Equivalent (GAE)/g dry weight and leaf extract 312.33 ± 9.81 mg GAE/g dry weight. In other respects, antioxidant activity related to the infusion method was determined for green fruit extract 172.51 ± 13.23 mg Trolox/L and leaf extract 569.16 ± 10.41 mg Trolox/L. Gas chromatography-mass spectrometry (GC-MS) analysis was used to identify the chemical composition of the extracts. The cytotoxicity levels of the extracts were evaluated on human adipose-derived mesenchymal stem cells (hAMSCs) using the commercially available XTT assay. Consequently, the results revealed the green fruit extract to have more cytotoxic activity then the leaf extract on hAMSCs.

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How to Cite
EFE, Burcu; GALATA, Yusuf Furkan; ARSLAN, Yavuz Emre. The Cytotoxicity Evaluation of Melia azedarach L. Extracts on Human Adipose-derived Mesenchymal Stem Cells. Hacettepe Journal of Biology and Chemistry, [S.l.], v. 46, n. 1, p. 121-128, feb. 2018. ISSN 1303-5002. Available at: <http://hjbc.hacettepe.edu.tr/index.php/hjbc/article/view/99>. Date accessed: 19 aug. 2018.
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