DEVELOPMENT OF A BIOSENSOR BASED ON MYRTLE (Myrtus Communis L.) TISSUE HOMOGENATE FOR VOLTAMMETRIC DETERMINATION OF EPINEPHRINE

Main Article Content

Adnan Ayna Erol Akyılmaz

Abstract

A novel plant tissue based biosensor was proposed for voltammetric determination of epinephrine (EP) in pharmaceutical samples. The tissue homogenate was immobilized by cross binding with glutaraldehyde on the glassy carbon. The polyphenol oxidase enzymes present in the fibers of a myrtle tree fruits maintained high bioactivity on this nanomaterial, catalyzing the oxidation of epinephrine to epinephrinequinone. Under optimized various operational parameters, the biosensor showed a linear response in the range of 10–100 µM. The limit of detection (LOD) was calculated as 3.2 × 10−6 mol L-1 (3.2 µM) (3σ per slope). The reproducibility, expressed as the relative standard deviation (RSD) for seven consecutive determinations of 5.0 × 10-5 mol L-1 EP was 4.6%. The biosensor had very good stability as it retained 70% activity after 11 days of storage in a phosphate buffer at 4°C. The applicability of this biosensor was demonstrated with the analysis of real samples and a good correlation was obtained between results acquired by the biosensor and those measured by spectrophotometric method. Such favorable results obtained with the myrtle tissue homogenate based biosensor, joined with the simplicity and low-cost of its preparation turns these procedures very attractive for EP quantification in pharmaceutical products.


 

Article Details

How to Cite
AYNA, Adnan; AKYILMAZ, Erol. DEVELOPMENT OF A BIOSENSOR BASED ON MYRTLE (Myrtus Communis L.) TISSUE HOMOGENATE FOR VOLTAMMETRIC DETERMINATION OF EPINEPHRINE. Hacettepe Journal of Biology and Chemistry, [S.l.], v. 46, n. 3, p. 321-328, sep. 2018. ISSN 1303-5002. Available at: <http://hjbc.hacettepe.edu.tr/index.php/hjbc/article/view/127>. Date accessed: 14 dec. 2018.
Section
Articles