Simultaneous Determination of Three Phenolic Compounds in Water Samples by Precolumn Derivatization Coupled with Reversed-Phase High Performance Liquid Chromatography

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BEDİHA AKMEŞE

Abstract

In this study, a sensitive and accurate method for simultaneous separation and determination of three phenolic compounds (phenol, m-cresol and resorcinol) in water by revesed-phase high performance liquid chromatography using uv-visible detection has been established. Pre-column derivatization with 4-aminoantipyrine is used for the seperation and determination phenol, m-cresol and resorcinol in water. The derivatives formed within 5 min. were extracted with chloroform and then analyzed by liquid chromatography with UV-visible detection at 440 nm. Chromatographic separation was performed using a reversed-phase column and acetonitrile-water (45:55 % v/v) as the mobile phase. The three derivatives were elueted in 13 min. The detection limits of phenol, m-cresol and resorcinol in a standard water sample were between 0.07 and 0.09 μg/L for 100 mL respectively. The recoveries of the derivatives from pure water were betweeen 97.1-102.3 % within relative standard deviations of 2.3-4.7. The method was applied to the analysis of phenols in diffrent water samples.

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How to Cite
AKMEŞE, BEDİHA. Simultaneous Determination of Three Phenolic Compounds in Water Samples by Precolumn Derivatization Coupled with Reversed-Phase High Performance Liquid Chromatography. Hacettepe Journal of Biology and Chemistry, [S.l.], v. 46, n. 2, p. 147-157, apr. 2018. ISSN 1303-5002. Available at: <http://hjbc.hacettepe.edu.tr/index.php/hjbc/article/view/108>. Date accessed: 17 july 2018.
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