Impedimetric Nanobiosensor for the Detection of Sequence-Selective DNA Hybridization

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Merve Muti İstek, 7393800 Mihrican Muti Erdem Arzum Erdem Gürsan

Abstract

5-amino-2-mercapto-1,3,4-thiadiazole (AMT) enriched gold nanoparticles (AuNPs) modified impedimetric sensors were developed for the electrochemical monitoring of sequence-selective DNA hybridization related to Hepatitis B virus (HBV). AMT-AuNP-PGEs presented more repeatable impedimetric responses and provided a suitable surface for more DNA binding onto AMT-AuNPs-PGE surface. The selectivity of DNA biosensor modified with AMT-AuNP was investigated in the presence of target DNA, or the other DNA sequences; e.g, noncomplementary (NC), or mismatch (MM) DNA sequences. The detection limit was calculated as 0.86 µg/mL.

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How to Cite
İSTEK, Merve Muti; ERDEM, Mihrican Muti; GÜRSAN, Arzum Erdem. Impedimetric Nanobiosensor for the Detection of Sequence-Selective DNA Hybridization. Hacettepe Journal of Biology and Chemistry, [S.l.], v. 46, n. 4, p. 495-503, jan. 2019. ISSN 1303-5002. Available at: <http://hjbc.hacettepe.edu.tr/index.php/hjbc/article/view/100>. Date accessed: 14 oct. 2019.
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